• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Partial Purification and Characterization î²-Galactosidase Lactobacillus Plantarum B123 Indigenos and Lactose Hydrolisis to Produce Low Lactose Ultra High Temperature Milk
【2h】

Partial Purification and Characterization î²-Galactosidase Lactobacillus Plantarum B123 Indigenos and Lactose Hydrolisis to Produce Low Lactose Ultra High Temperature Milk

机译：部分纯化和表征β-半乳糖苷酶植物乳杆菌B123本地和乳糖水解生产低乳糖超高温牛奶

页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

β-Galactosidase is enzyme which hidrolyze lactose to glucose and galactose. This enzyme is used in production low lactose milk for consumption human which have lactose intolerance. Partial purification of β-galactosidase is important to be conducted to increase β-galactosidase activity in order to its hydrolysis potency on UHT milk lactose increased.This research was aimed to production by partially purification and characterization indigenous β-galactosidase from Lactobacillus plantarum B123, and lactose hydrolysis for production low lactose UHT milk. Partially purification were precipitation following dialysis. Characterization included optimazion and stabilization of enzyme, while lactose hydrolisis for production low lactose UHT milk was detected by enzymatic GOD-POD kit. The results showed that production of β-galactosidase by using partial purification increased from 21.51 ± 0.23 U/mL (crude) to 106.34 ± 0.56 U/mL (dialysis). The optimum crude β-galactosidase activity was reached in precipitation by using 60 % ammonium sulphate. The purity of crude β-galactosidase increased 3.71 times after precipitation, and 14.28 times after dialysis. Characterization of β-galactosidase showed that optimum activities of crude and dialyzed β-galactosidase were at pH 6.5 and 50 oC, respectively. Stability of crude β-galactosidase incubated for 1 h were at pH: 5.0-8.5 and 25-50 °C. Specific activity of crude β-galactosidase was 15.05 U/mg protein, while that dialyzed β-galactosidase was 109.58 U/mg protein. Lactose hidrolysis to produce low lactose UHT milk showed that glucose concentration increased with the increase of hidrolysis time. Time needed to hidrolyze lactose 50 % with 4.8 U/mL β-galactosidase at 50°C was 6.08 h. In conclusion that indigenous β-galactosidase from Lactobacillus plantarum B123 purified partially can be used as lactose hidrolyzer in production of low lactose UHT milk.
机译：β-半乳糖苷酶是将乳糖水解成葡萄糖和半乳糖的酶。该酶用于生产乳糖不耐症的低乳糖牛奶以供食用。部分纯化β-半乳糖苷酶很重要，以提高β-半乳糖苷酶活性，以使其对超高温灭菌牛奶乳糖的水解能力增强。这项研究旨在通过部分纯化和鉴定植物乳杆菌B123中的本地β-半乳糖苷酶来进行生产。乳糖水解生产低乳糖超高温灭菌牛奶。部分纯化是透析后的沉淀。表征包括酶的优化和稳定化，而通过酶促GOD-POD试剂盒检测了用于生产低乳糖UHT牛奶的乳糖水解。结果表明，通过使用部分纯化法生产的β-半乳糖苷酶从21.51±0.23 U / mL（原油）增加到106.34±0.56 U / mL（透析）。最佳的粗β-半乳糖苷酶活性在沉淀作用中达到使用60％硫酸铵。沉淀后，粗β-半乳糖苷酶的纯度增加了3.71倍，透析后增加了14.28倍。表征的β-半乳糖苷酶表明，粗和透析的β-半乳糖苷酶的最佳活性分别在pH 6.5和50 oC。孵育1小时的粗β-半乳糖苷酶的稳定性在pH：5.0-8.5和25-50°C。粗β-半乳糖苷酶的比活为15.05 U / mg蛋白，而透析的β-半乳糖苷酶为109.58 U / mg蛋白。乳糖水解产生低乳糖UHT牛奶显示葡萄糖浓度随水解时间的增加而增加。在50°C下用4.8 U / mLβ-半乳糖苷酶水解乳糖50％所需的时间为6.08小时。结论是，部分纯化的植物乳杆菌B123中的本地β-半乳糖苷酶可以用作低乳糖超高温灭菌牛奶生产中的乳糖水解仪。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号